As a guest user you are not logged in or recognized by your IP address. You have
access to the Front Matter, Abstracts, Author Index, Subject Index and the full
text of Open Access publications.
Introduction: Longitudinal growth of long bone is a process of new bone formation involving progressive endochondral ossification which occurs at the growth plate before skeletal maturity. It is documented that melatonin plays an important role in bone formation and metabolism. The role of melatonin in human growth plate chondrocytes (GPCs), however, is relatively poorly studied.
Objectives: To investigated the effect of melatonin on the proliferation and differentiation of human GPCs.
Materials and Methods: Human fetus growth plates were harvested for GPCs primary culture. GPCs were treated with serial concentrations of melatonin for three days, and then the cell proliferation was measured by number of cells with MTT assay. For the effect of melatonin on GPCs differentiation, GPCs at confluence were treated with melatonin for two weeks for the examination of mRNA expression of collagen type X and alkaline phosphatase by real time PCR.
Results: The GPCs maintained in polygonal-shape during culture and became spindle-shape when full confluence. The proliferation of GPCs was significantly reduced by melatonin at both physiological and pharmacological dosages. Melatonin also reduced the mRNA level of collagen type X and alkaline phosphatase in differentiated GPCs, indicating its retarding effect on the differentiation of GPCs.
Conclusion: The present results infer that melatonin is involved in the modulation of the activity of human GPCs
Significance: Melatonin might play an important role in the regulation of endochondral ossification.
This website uses cookies
We use cookies to provide you with the best possible experience. They also allow us to analyze user behavior in order to constantly improve the website for you. Info about the privacy policy of IOS Press.
This website uses cookies
We use cookies to provide you with the best possible experience. They also allow us to analyze user behavior in order to constantly improve the website for you. Info about the privacy policy of IOS Press.