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Introduction: Decreased bone mineral density (BMD) among scoliosis patients with neurofibromatosis type1 (NF1) has been reported. Our previous study showed high proliferation rate but lower differentiation activity of the osteoblasts from scoliosis with NF1. Bone morphogenetic protein-2 (BMP-2) is one of the central regulators of osteoblast differentiation. Whether BMP-2 was involved in the pathogenesis of low BMD and low differentiation activity of osteoblasts in scoliosis with NF1 has not been reported.
Objectives: To compare the expression of BMP-2 in osteoblast from scoliosis with NF1 and control subjects.
Materials and Methods: Cancellous bone biopsies were harvested from ten NF1 scoliosis patients and 9 age-matched control for osteoblasts primary culture. BMD of the lumbar spine and proximal femur was measured using dual energy X-ray absorptiometry. Osteoblasts were harvested at the end of 2nd passage for mRNA and protein extraction. The expression of BMP-2 was detected by RT-PCR and Western blotting, respectively. Comparison was made between NF1 and control subjects by non-parametric analysis.
Results: BMD was obviously lower in NF1 group than that in control group (p<0.01). Neither mRNA nor protein expression of BMP-2 in osteoblasts from scoliosis with NF1 was significantly difference from that in control subjects (P>0.05).
Conclusion: Although low bone mass was found in patients with NF1 scoliosis, there was no abnormal expression of BMP-2 in osteoblasts.
Significance: BMP-2 might not be involved in the regulation of abnormal proliferation and differentiation of osteoblasts and low bone mass in patients with NF1 scoliosis.
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